Selected Publications

2024

Crhak Khaitova L, Mikulkova P, Pecinkova J, Kalidass M, Heckmann S, Lermontova I, Riha K. Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis. Elife. 2024 Apr 17;12:RP90253. doi: 10.7554/eLife.90253. PMID: 38629825

Volkava D, Riha K. Growing old while staying young : The unique mechanisms that defy aging in plants. EMBO Rep. 2024 Jan 26. doi: 10.1038/s44319-024-00062-4. Online ahead of print.PMID: 38279018

2023

Fulneček J, Klimentová E, Cairo A, Bukovcakova SV, Alexiou P, Prokop Z, Riha K. The SAP domain of Ku facilitates its efficient loading onto DNA ends. Nucleic Acids Res. 2023 Nov 27;51(21):11706-11716. doi: 10.1093/nar/gkad850.PMID: 37850645

Tanasa S, Shukla N, Cairo A, Ganji RS, Mikulková P, Valuchova S, Raxwal VK, Capitao C, Schnittger A, Zdráhal Z, Riha K. A complex role of Arabidopsis CDKD;3 in meiotic progression and cytokinesis. Plant Direct. 2023 Mar 6;7(3):e477. doi: 10.1002/pld3.477. eCollection 2023 Mar.PMID: 36891158 

Raxwal VK, Riha K. The biological functions of nonsense-mediated mRNA decay in plants: RNA quality control and beyond. Biochem Soc Trans. 2023 Feb 27;51(1):31-39. doi: 10.1042/BST20211231.PMID: 36695509

Ahmadli U, Kalidass M, Khaitova LC, Fuchs J, Cuacos M, Demidov D, Zuo S, Pecinkova J, Mascher M, Ingouff M, Heckmann S, Houben A, Riha K, Lermontova I. High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis. Plant Commun. 2023 May 8;4(3):100507. doi: 10.1016/j.xplc.2022.100507. Epub 2022 Dec 20.PMID: 36540022

2022

Cairo A, Vargova A, Shukla N, Capitao C, Mikulkova P, Valuchova S, Pecinkova J, Bulankova P, Riha K. Meiotic exit in Arabidopsis is driven by P-body-mediated inhibition of translation. Science. 2022 Aug 5;377(6606):629-634. doi: 10.1126/science.abo0904. Epub 2022 Aug 4.PMID: 35926014

Raxwal VK, Singh S, Agarwal M, Riha K. Transcriptional and post-transcriptional regulation of young genes in plants. BMC Biol. 2022 Jun 9;20(1):134. doi: 10.1186/s12915-022-01339-7.PMID: 35676681 

Valuchova S, Mikulkova P, Pecinkova J, Riha K. Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy. Methods Mol Biol. 2022;2484:93-105. doi: 10.1007/978-1-0716-2253-7_8.PMID: 35461447

Romeiro Motta M, Zhao X, Pastuglia M, Belcram K, Roodbarkelari F, Komaki M, Harashima H, Komaki S, Kumar M, Bulankova P, Heese M, Riha K, Bouchez D, Schnittger A. B1-type cyclins control microtubule organization during cell division in Arabidopsis. EMBO Rep. 2022 Jan 5;23(1):e53995. doi: 10.15252/embr.202153995. Epub 2021 Dec 9.PMID: 34882930 

2021

Watson JM, Trieb J, Troestl M, Renfrew K, Mandakova T, Fulnecek J, Shippen DE, Riha K.  A hypomorphic allele of telomerase uncovers the minimal functional length of telomeres in Arabidopsis. Genetics. 
2021 Oct 2;219(2):iyab126. doi: 10.1093/genetics/iyab126. PMID: 34849882 

Capitao C, Tanasa S, Fulnecek J, Raxwal VK, Akimcheva S, Bulankova P, Mikulkova P, Crhak Khaitova L, Kalidass M, Lermontova I, Mittelsten Scheid O, Riha K. A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis. PLoS Genet. 2021 Sep 30;17(9):e1009779. doi: 10.1371/journal.pgen.1009779. eCollection 2021 Sep. PMID: 34591845

Fulneček J, Říha K. High-Throughput Protein-Nucleic Acid Interaction Assay Based on Protein-Induced Fluorescence Enhancement. Methods Mol Biol. 2021;2209:109-117. doi: 10.1007/978-1-0716-0935-4_7.PMID: 33201465​

2020

Valuchova S, Mikulkova P, Pecinkova J, Klimova J, Krumnikl M, Bainar P, Heckmann S, Tomancak P, Riha K. Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy. Elife. 2020 Feb 11;9. pii: e52546. doi: 10.7554/eLife.52546. PMID: 32041682

Vivek K. Raxwal, Craig G. Simpson, Jiradet Gloggnitzer, Juan Carlos Entinze, Wenbin Guo, Runxuan Zhang, John W.S. Brown,  Karel Riha. Nonsense mediated RNA decay factor UPF1 is critical for post-transcriptional and translational gene regulation in Arabidopsis. bioRxiv

 
Raxwal VK, Simpson CG, Gloggnitzer J, Entinze JC, Guo W, Zhang R, Brown JWS, Riha K., Nonsense-mediated RNA Decay Factor UPF1 is Critical for Post-transcriptional and Post-translational Gene Regulation in Arabidopsis. Plant Cell. 2020 Jul 14:tpc.00244.2020. doi: 10.1105/tpc.20.00244. Online ahead of print.PMID: 32665305

2019

Javorka P, Raxwal VK, Najvarek J, Riha K. artMAP: A user-friendly tool for mapping ethyl methanesulfonate-induced mutations in Arabidopsis. Plant Direct. 2019 Jun 11;3(6):e00146. doi: 10.1002/pld3.146. eCollection 2019 Jun. PMID: 31245783

Eberhard S, Valuchova S, Ravat J, Fulneček J, Jolivet P, Bujaldon S, Lemaire SD, Wollman FA, Teixeira MT, Riha K, Xu Z.Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants. Life Sci Alliance. 2019 Jun 3;2(3). pii: e201900315. doi: 10.26508/lsa.201900315. Print 2019 Jun. PMID: 31160377.

Valuchova S, Mikulkova P, Pecinkova J, Klimova J, Krumnikl M, Bainar P, Heckmann S, Tomancak P, Riha K. Imaging plant germline differentiation within Arabidopsis flower by light sheet microscopy. bioRxiv, 774299 

2018

Capitao C, Shukla N, Wandrolova A, Mittelsten Scheid O, Riha K. Functional Characterization of SMG7 Paralogs in Arabidopsis thaliana. Front Plant Sci. 2018 Nov 6;9:1602. doi: 10.3389/fpls.2018.01602. eCollection 2018. PMID: 30459790.

Javorka P, Raxwal VK, Najvarek J, Riha K. artMAP: a user-friendly tool for mapping EMS-induced mutations in Arabidopsis bioRxiv, 414433.

2017

Valuchova S, Fulnecek J, Prokop Z, Stolt-Bergner P, Janouskova E, Hofr C, Riha K. Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer. Plant Cell. 2017 Jun;29(6):1533-1545. doi: 10.1105/tpc.17.00064. Epub 2017 Jun 5. PMID: 28584163.

2016

Valuchova S, Fulnecek J, Petrov AP, Tripsianes K, Riha K. A rapid method for detecting protein-nucleic acid interactions by protein induced fluorescence enhancement. Sci Rep. 2016 Dec 23;6:39653. doi: 10.1038/srep39653. PubMed PMID: 28008962.

Watson JM, Platzer A, Kazda A, Akimcheva S, Valuchova S, Nizhynska V, Nordborg M, Riha K. Germline replications and somatic mutation accumulation are independent of vegetative life span in Arabidopsis. Proc Natl Acad Sci U S A. 2016 Oct 25;113(43):12226-12231. PubMed PMID: 27729523; PubMed Central PMCID: PMC5087024.

Raxwal VK, Riha K. Nonsense mediated RNA decay and evolutionary capacitance. Biochim Biophys Acta. 2016 Dec;1859(12):1538-1543. doi: 10.1016/j.bbagrm.2016.09.001. Review. PubMed PMID: 27599370.

Valuchova, S., Derboven, E. and Riha, K. Analysis of Telomeric G-overhangs by in-Gel Hybridization. Bio-protocol. 2016 Apr; 6(7): e1775.  

Fulcher N, Riha K. Using Centromere Mediated Genome Elimination to Elucidate the Functional Redundancy of Candidate Telomere Binding Proteins in Arabidopsis thaliana. Front Genet. 2016 Jan 5;6:349. doi: 10.3389/fgene.2015.00349. PubMed PMID: 26779251; PubMed Central PMCID: PMC4700174.

Kazda A, Akimcheva S, Watson JM, Riha K. Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis. Methods Mol Biol. 2016;1370:169-82. doi: 10.1007/978-1-4939-3142-2_13. PubMed PMID: 26659962.

2015

Fulcher N, Teubenbacher A, Kerdaffrec E, Farlow A, Nordborg M, Riha K. Genetic architecture of natural variation of telomere length in Arabidopsis thaliana. Genetics. 2015 Feb;199(2):625-35. doi: 10.1534/genetics.114.172163. PubMed PMID: 25488978; PubMed Central PMCID: PMC4317667.

2014

Derboven E, Ekker H, Kusenda B, Bulankova P, Riha K. Role of STN1 and DNA polymerase α in telomere stability and genome-wide replication in Arabidopsis. PLoS Genet. 2014 Oct 9;10(10):e1004682. doi: 10.1371/journal.pgen.1004682. PubMed PMID: 25299252; PubMed Central PMCID: PMC4191939.

Gloggnitzer J, Akimcheva S, Srinivasan A, Kusenda B, Riehs N, Stampfl H, Bautor J, Dekrout B, Jonak C, Jiménez-Gómez JM, Parker JE, Riha K. Nonsense-mediated mRNA decay modulates immune receptor levels to regulate plant antibacterial defense. Cell Host Microbe. 2014 Sep 10;16(3):376-90. doi: 10.1016/j.chom.2014.08.010. PubMed PMID: 25211079.

Plecenikova A, Slaninova M, Riha K. Characterization of DNA repair deficient strains of Chlamydomonas reinhardtii generated by insertional mutagenesis. PLoS One. 2014 Aug 21;9(8):e105482. doi: 10.1371/journal.pone.0105482. PubMed PMID: 25144319; PubMed Central PMCID: PMC4140758.

Göhring J, Fulcher N, Jacak J, Riha K. TeloTool: a new tool for telomere length measurement from terminal restriction fragment analysis with improved probe intensity correction. Nucleic Acids Res. 2014 Feb;42(3):e21. doi: 10.1093/nar/gkt1315. PubMed PMID: 24366880; PubMed Central PMCID: PMC3919618.

Fulcher N, Derboven E, Valuchova S, Riha K. If the cap fits, wear it: an overview of telomeric structures over evolution. Cell Mol Life Sci. 2014 Mar;71(5):847-65. doi: 10.1007/s00018-013-1469-z. Review. PubMed PMID: 24042202.

2013

Bulankova P, Akimcheva S, Fellner N, Riha K. Identification of Arabidopsis meiotic cyclins reveals functional diversification among plant cyclin genes. PLoS Genet. 2013 May;9(5):e1003508. doi: 10.1371/journal.pgen.1003508. PubMed PMID: 23671425; PubMed Central PMCID: PMC3649987.

2012

Kazda A, Zellinger B, Rössler M, Derboven E, Kusenda B, Riha K. Chromosome end protection by blunt-ended telomeres. Genes Dev. 2012 Aug 1;26(15):1703-13. doi: 10.1101/gad.194944.112. PubMed PMID: 22810623; PubMed Central PMCID: PMC3418588.

Riehs-Kearnan N, Gloggnitzer J, Dekrout B, Jonak C, Riha K. Aberrant growth and lethality of Arabidopsis deficient in nonsense-mediated RNA decay factors is caused by autoimmune-like response. Nucleic Acids Res. 2012 Jul;40(12):5615-24. doi: 10.1093/nar/gks195. PubMed PMID: 22379136; PubMed Central PMCID: PMC3384318.

2011

Watson JM, Riha K. Telomeres, aging, and plants: from weeds to Methuselah - a mini-review. Gerontology. 2011;57(2):129-36. doi: 10.1159/000310174. Review. PubMed PMID: 20375491.

2010

Bulankova P, Riehs-Kearnan N, Nowack MK, Schnittger A, Riha K. Meiotic progression in Arabidopsis is governed by complex regulatory interactions between SMG7, TDM1, and the meiosis I-specific cyclin TAM. Plant Cell. 2010 Nov;22(11):3791-803. doi: 10.1105/tpc.110.078378. PubMed PMID: 21119056; PubMed Central PMCID: PMC3015126.

Watson JM, Riha K. Comparative biology of telomeres: where plants stand. FEBS Lett. 2010 Sep 10;584(17):3752-9. doi: 10.1016/j.febslet.2010.06.017. Review. PubMed PMID: 20580356; PubMed Central PMCID: PMC3767043.

Vrbsky J, Akimcheva S, Watson JM, Turner TL, Daxinger L, Vyskot B, Aufsatz W, Riha K. siRNA-mediated methylation of Arabidopsis telomeres. PLoS Genet. 2010 Jun 10;6(6):e1000986. doi: 10.1371/journal.pgen.1000986. PubMed PMID: 20548962; PubMed Central PMCID: PMC2883606.

2008

Akimcheva S, Zellinger B, Riha K. Genome stability in Arabidopsis cells exhibiting alternative lengthening of telomeres. Cytogenet Genome Res. 2008;122(3-4):388-95. doi: 10.1159/000167827. PubMed PMID: 19188710.

Riehs N, Akimcheva S, Puizina J, Bulankova P, Idol RA, Siroky J, Schleiffer A, Schweizer D, Shippen DE, Riha K. Arabidopsis SMG7 protein is required for exit from meiosis. J Cell Sci. 2008 Jul 1;121(Pt 13):2208-16. doi: 10.1242/jcs.027862. PubMed PMID: 18544632.

2007

Zellinger B, Akimcheva S, Puizina J, Schirato M, Riha K. Ku suppresses formation of telomeric circles and alternative telomere lengthening in Arabidopsis. Mol Cell. 2007 Jul 6;27(1):163-9. PubMed PMID: 17612498.

Zellinger B, Riha K. Composition of plant telomeres. Biochim Biophys Acta. 2007 May-Jun;1769(5-6):399-409. Review. PubMed PMID: 17383025.

2006

2005

2004

2003

Riha K, Shippen DE. Telomere structure, function and maintenance in Arabidopsis. Chromosome Res. 2003;11(3):263-75. Review. PubMed PMID: 12769293.

Riha K, Shippen DE. Ku is required for telomeric C-rich strand maintenance but not for end-to-end chromosome fusions in Arabidopsis. Proc Natl Acad Sci U S A. 2003 Jan 21;100(2):611-5. PubMed PMID: 12511598; PubMed Central PMCID: PMC141044.

2002

2001

2000

1998